Gel-Electrophoresis and Its Applications
نویسنده
چکیده
Positive or negative electrical charges are frequently associated with biomolecules. When placed in an electric field, charged biomolecules move towards the electrode of opposite charge due to the phenomenon of electrostatic attraction. Electrophoresis is the separation of charged molecules in an applied electric field. The relative mobility of individual molecules depends on several factors. The most important of which are net charge, charge/mass ratio, molecular shape and the temperature, porosity and viscosity of the matrix through which the molecule migrates. Complex mixtures can be separated to very high resolution by this process (Sheehan, D.; 2000).
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